J Virol. 2008 January; 82(1): 451460. Published online 2007 October 24. doi: 10.1128/JVI.01251-07.PMCID: PMC2224397 Copyright Â© 2008, American coalition for MicrobiologyIntra cadreular Restriction of a Productive Noncytopathic Coronavirus Infection Olga Slobodskaya, Alexander Laarman,# and Willy J. M. Spaan*molecular(a) Virology Laboratory, discussion section of medical checkup Microbiology, Center of Infectious Diseases, Leiden University medical exam Center, P.O. boxful 9600, 2300 RC Leiden, The Netherlands*Corresponding author. Mailing address: Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, LUMC E4-P, P.O. Box 9600, 2300 RC Leiden, The Netherlands. Phone: 31715261652. Fax: 31715261667. E-mail: firstname.lastname@example.org # map address: Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.Received June 8, 2007; legitimate October 13, 2007.Â· OTHER SECTIONS? OABSTRACT OMATERIALS AND METHODSORESULTSODISCUSSIONOREFERENCESABSTRACTVirus infection in vitro can all impart in a cytopathic effect (CPE) or survive without perceptible changes in infected cells (noncytopathic infection). We are interested in agreement the mechanisms controlling the impact of coronavirus infection on emcee cells.
To this end, we compared a productive, noncytopathic infection of murine hepatitis virus (MHV) strain A59 in the fibroblastlike cell line NIH 3T3 with cytopathic MHV infections. Infected NIH 3T3 cells could be cultured for up to 4 weeks without apparent CPE and yet produce virus at 107 to 108 PFU/ml. ut ilize flow cytometry, we demonst treasured t! hat NIH 3T3 cells expressed as frequently MHV receptor CEACAM1 as other cell lines which die from MHV infection. In contrast, utilize quantitative reverse transcription-PCR and metabolic labeling of ribonucleic acid, we found that the pose of viral RNA amplification in NIH 3T3 cells was lower than the rate in cells in which MHV induces a CPE. The rate of cellular RNA price reduction in...If you want to get a full essay, rove it on our website: OrderCustomPaper.com
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